Kizawa K, Naganuma K, Murakami U
Biochemistry Laboratory, Kanebo Ltd, Odawara, Japan.
J Dairy Res. 1995 Nov;62(4):587-92. doi: 10.1017/s0022029900031319.
Peptides that inhibit calmodulin-dependent cyclic nucleotide phosphodiesterase were isolated from a pepsin digest of alpha-casein. Analysis of these peptides showed that they corresponded to the alpha S2-casein sequences 164-179 (Leu-Lys-Lys-Ile-Ser-Gln-Arg-Tyr-Gln-Lys-Phe-Ala-Leu-Pro-Gln-Tyr). 183-206 (Val-Tyr-Gln-His-Gln-Lys-Ala-Met-Lys-Pro-Trp-Ile-Gln-Pro-Lys-Thr-Lys-Val -Ile- Pro-Tyr-Val-Arg-Tyr) and 183-207 (C-terminus, Val-Tyr-Gln-His-Gln-Lys-Ala-Met-Lys-Pro-Trp-Ile- Gln-Pro-Lys-Thr-Lys-Val-Ile-Pro-Tyr-Val-Arg-Tyr-Leu). These peptides inhibited calmodulin-induced cyclic nucleotide phosphodiesterase activity over the range 1-50 microM without affecting the basal enzyme activity. These results demonstrated that the affinities of these peptides for calmodulin are comparable to the affinities of certain endogenous neurohormones and proteins that interact with calmodulin.
从α-酪蛋白的胃蛋白酶消化物中分离出了抑制钙调蛋白依赖性环核苷酸磷酸二酯酶的肽段。对这些肽段的分析表明，它们对应于αS2-酪蛋白序列164 - 179（亮氨酸-赖氨酸-赖氨酸-异亮氨酸-丝氨酸-谷氨酰胺-精氨酸-酪氨酸-谷氨酰胺-赖氨酸-苯丙氨酸-丙氨酸-亮氨酸-脯氨酸-谷氨酰胺-酪氨酸）、183 - 206（缬氨酸-酪氨酸-谷氨酰胺-组氨酸-谷氨酰胺-赖氨酸-丙氨酸-甲硫氨酸-赖氨酸-脯氨酸-色氨酸-异亮氨酸-谷氨酰胺-脯氨酸-赖氨酸-苏氨酸-赖氨酸-缬氨酸-异亮氨酸-脯氨酸-酪氨酸-缬氨酸-精氨酸-酪氨酸）和183 - 207（C末端，缬氨酸-酪氨酸-谷氨酰胺-组氨酸-谷氨酰胺-赖氨酸-丙氨酸-甲硫氨酸-赖氨酸-脯氨酸-色氨酸-异亮氨酸-谷氨酰胺-脯氨酸-赖氨酸-苏氨酸-赖氨酸-缬氨酸-异亮氨酸-脯氨酸-酪氨酸-缬氨酸-精氨酸-酪氨酸-亮氨酸）。这些肽段在1 - 50微摩尔范围内抑制钙调蛋白诱导的环核苷酸磷酸二酯酶活性，而不影响基础酶活性。这些结果表明，这些肽段对钙调蛋白的亲和力与某些与钙调蛋白相互作用的内源性神经激素和蛋白质的亲和力相当。
