Kobayashi M, Akiyama Y, Tomiuga T, Hara K, Nakamura T
Department of Applied Drug Research, Eisai Co., Ltd., Japan.
Nihon Seikeigeka Gakkai Zasshi. 1995 Nov;69(11):1170-81.
An image analyzer which combines a digitizer and a computer is widely employed in bone histomorphometrical evaluation. With this method, however, all regions of determination are traced manually so that considerable time is consumed when many preparations are measured. To resolve this problem, we have developed an automatic program for rapid and quantitative measurement of many bone histomorphometrical parameters using a computed color image analyzer. The bone volume (BV), tissue volume (TV), osteoid surface (OS), and the bone surface (BS) of rat tibia metaphysis were measured by this automatic method, and by the manual method using an image analyzer. The correlation coefficient between both methods in measuring BV/TV was 0.98 and in measuring OS/BS was 0.96. The reproducibility and accuracy of both methods for these parameters were similar. The time required for the automatic method was much shorter than that for the manual method. Various parameters of the tibial metaphysis, lumbar vertebral body, and femoral mid-diaphysis in ovariectomized rat (Fischer) measured by this method were in good agreement with values reported previously.
一种结合了数字化仪和计算机的图像分析仪被广泛应用于骨组织形态计量学评估。然而，使用这种方法时，所有测定区域都需要手动追踪，因此在测量大量标本时会消耗大量时间。为了解决这个问题，我们开发了一个自动程序，使用计算机彩色图像分析仪快速定量测量许多骨组织形态计量学参数。用这种自动方法以及使用图像分析仪的手动方法测量大鼠胫骨近端骨骺的骨体积（BV）、组织体积（TV）、类骨质表面（OS）和骨表面（BS）。两种方法测量BV/TV的相关系数为0.98，测量OS/BS的相关系数为0.96。两种方法对这些参数的重复性和准确性相似。自动方法所需的时间比手动方法短得多。用这种方法测量的去卵巢大鼠（Fischer）胫骨近端骨骺、腰椎椎体和股骨干中部的各种参数与先前报道的值高度一致。
