Dreyer T, Popella C, Hinrichs B, Bohle R M, Pohlmann U, Schulz A, Glanz H
Institute of Pathology, Justus Liebig University, Giessen, Germany.
J Pathol. 1995 Dec;177(4):385-93. doi: 10.1002/path.1711770409.
In order to detect early precancerous changes which do not involve the whole thickness of the epithelium, we used a novel image analysing program based on an IBAS system (Kontron, Germany) to determine nuclear DNA content (NC) as well as average nuclear area (NA) and variation of nuclear area (VA), in the entire epithelium and in three sublayers, parabasal, intermediate, and superficial. DNA aneuploidy was found in only half of the cases classified as 'high-grade' (HG) lesions, comprising carcinoma in situ (CIS) and severe epithelial dysplasias (EDIII), and was chiefly demonstrable in the parabasal third of the epithelium. The other lesions were DNA euploid. HG lesions showed highly significant increases of NA and VA at the lower levels of the epithelium when compared with 'low-grade' (LG) lesions comprising moderate and mild epithelial dysplasias (EDII and EDI). Our data show that the combination of multiparameter image analysis with conventional morphology assists in the objective grading of precancerous lesions and permits the reliable detection of high-grade lesions.
为了检测未累及上皮全层的早期癌前病变，我们使用了基于IBAS系统（德国康强电子公司）的新型图像分析程序，以确定整个上皮以及基底层、中层和表层三个亚层中的核DNA含量（NC）、平均核面积（NA）和核面积变异（VA）。在仅一半被归类为“高级别”（HG）病变的病例中发现了DNA非整倍体，这些病变包括原位癌（CIS）和重度上皮发育异常（EDIII），且主要在上皮的基底层三分之一处得以证实。其他病变为DNA整倍体。与包括中度和轻度上皮发育异常（EDII和EDI）的“低级别”（LG）病变相比，HG病变在上皮较低水平处的NA和VA显著增加。我们的数据表明，多参数图像分析与传统形态学相结合有助于对癌前病变进行客观分级，并能可靠地检测出高级别病变。
