口腔异时性癌前病变及后续癌中的核DNA含量与p53免疫染色
Nuclear DNA content and p53 immunostaining in metachronous preneoplastic lesions and subsequent carcinomas of the oral cavity.
作者信息
Högmo A, Munck-Wikland E, Kuylenstierna R, Lindholm J, Auer G
机构信息
Department of Oto-rhino-laryngology, Danderyd Hospital, Stockholm, Sweden.
出版信息
Head Neck. 1996 Sep-Oct;18(5):433-40. doi: 10.1002/(SICI)1097-0347(199609/10)18:5<433::AID-HED6>3.0.CO;2-6.
BACKGROUND
Clinical evaluation of preneoplastic lesions of the oral cavity is difficult. Histopathologic grading of dysplasias shows large variability and does not give reliable information concerning the risk for progression to cancer.
METHODS
DNA image cytometry and p53 immunostaining were performed to describe the pattern of DNA aberration and p53 overexpression in confined preneoplastic lesions and in the subsequent carcinomas developing at the same site in 20 patients.
RESULTS
Hyperplastic and/or inflammatory lesions showed a diploid DNA pattern in 81% of the cases and 23% were p53-positive. Dysplastic preneoplastic lesions showed a nondiploid/ aneuploid DNA pattern in 73% and 64% were p53-positive. The subsequent invasive carcinomas were nondiploid/aneuploid in 86% and p53-positive in 69% of cases.
CONCLUSIONS
Analysis of nuclear DNA content and p53 immunostaining appears to be useful as an adjunct to histopathology in the evaluation of true precancerous lesions.
背景
口腔癌前病变的临床评估具有难度。发育异常的组织病理学分级显示出很大的变异性，并且无法提供有关进展为癌症风险的可靠信息。
方法
对20例患者的局限性癌前病变及其在同一部位随后发生的癌进行DNA图像细胞术和p53免疫染色，以描述DNA畸变和p53过表达模式。
结果
增生性和/或炎性病变在81%的病例中显示二倍体DNA模式，23%为p53阳性。发育异常的癌前病变在73%的病例中显示非二倍体/非整倍体DNA模式，64%为p53阳性。随后的浸润性癌在86%的病例中为非二倍体/非整倍体，69%为p53阳性。
结论
在评估真正的癌前病变时，核DNA含量分析和p53免疫染色似乎可作为组织病理学的辅助手段。
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