Hofrichter M, Bublitz F, Fritsche W
Friedrich-Schiller-Universität Jena, Institut für Mikrobiologie, Germany.
J Basic Microbiol. 1995;35(5):303-13. doi: 10.1002/jobm.3620350505.
o-Cresol induced glucose-grown resting mycelia of Penicillium frequentans Bi 7/2 (ATCC-number: 96048) immediately oxidized o-cresol and other phenols. After precultivation on glucose and phenol degradation started after a lag-phase of 24 hours. Metabolites of o-cresol metabolism were methylhydroquinone, methyl-p-benzoquinone, 2-methyl-5-hydroxyhydroquinone and 2-methyl-5-hydroxy-p-benzoquinone. The initial reaction is probably catalyzed by a NADPH dependent hydroxylase which is specific for o-cresol. The metabolism of 2,6-dimethylphenol (2,6-xylenol) occurred via 2,6-dimethylhydroquinone, 2,6-dimethyl-p-benzoquinone, 2,6-dimethyl-3-hydroxyhydroquinone, 2,6-dimethyl-3-hydroxy-p-benzoquinone and 3-methyl-2-hydroxybenzoic acid.
邻甲酚可诱导频繁青霉Bi 7/2（ATCC编号：96048）在以葡萄糖为培养基生长的静止菌丝体立即氧化邻甲酚和其他酚类物质。在葡萄糖上预培养后，经过24小时的延迟期开始酚类物质降解。邻甲酚代谢产物有甲基对苯二酚、甲基对苯醌、2-甲基-5-羟基对苯二酚和2-甲基-5-羟基对苯醌。初始反应可能由一种对邻甲酚具有特异性的NADPH依赖性羟化酶催化。2,6-二甲基苯酚（2,6-二甲酚）的代谢途径为2,6-二甲基对苯二酚、2,6-二甲基对苯醌、2,6-二甲基-3-羟基对苯二酚、2,6-二甲基-3-羟基对苯醌和3-甲基-2-羟基苯甲酸。
