Breckenridge L J, Wilson R J, Connolly P, Curtis A S, Dow J A, Blackshaw S E, Wilkinson C D
Laboratory of Cell Biology, University of Glasgow, United Kingdom.
J Neurosci Res. 1995 Oct 1;42(2):266-76. doi: 10.1002/jnr.490420215.
We describe fabrication methods and the characterisation and use of extracellular microelectrode arrays for the detection of action potentials from neurons in culture. The 100 microns2 platinised gold microelectrodes in the 64 electrode array detect the external current which flows during an action potential with S:N ratios of up to 500:1, giving a maximum recorded signal of several millivolts. The performance of these electrodes is enhanced if good sealing of the cells over the electrodes is obtained and further enhanced if the electrodes and the cells lie in a deep groove in the substratum. The electrodes can be used for both recording and stimulation of activity in cultured neurons and for recording from multiple sites on a single cell. The use of such electrodes to obtain recordings from invertebrate neurons is described. The particular advantages of these electrodes, their long term stability, non-invasive nature, high packing density, and utility in stimulation, are demonstrated.
我们描述了用于检测培养神经元动作电位的细胞外微电极阵列的制造方法、特性及应用。64电极阵列中的100平方微米镀铂金微电极可检测动作电位期间流动的外部电流，信噪比高达500:1，记录到的最大信号可达几毫伏。如果能使细胞在电极上良好密封，这些电极的性能会得到增强；若电极和细胞位于基质中的深槽内，性能会进一步增强。这些电极可用于记录和刺激培养神经元的活动，也可用于从单个细胞的多个位点进行记录。本文还描述了使用此类电极从无脊椎动物神经元获取记录的情况。展示了这些电极的特殊优势，即长期稳定性、非侵入性、高封装密度以及在刺激方面的实用性。
