Kobayashi I, Hamasaki Y, Sato R, Zaitu M, Muro E, Yamamoto S, Ichimaru T, Miyazaki S
Department of Pediatrics, Saga Medical School, Japan.
J Ethnopharmacol. 1995 Aug 11;48(1):33-41. doi: 10.1016/0378-8741(95)01280-q.
Saiboku-To, a mixture of extracts from 10 medicinal herbs, has been used for the treatment of bronchial asthma in Japan. Inhibitory action of this drug on arachidonate 5-lipoxygenase (5-LO) metabolism in rat basophilic leukemia cells (RBL-1 cells) was examined. Saiboku-To significantly inhibited calcium ionophore-stimulated synthesis of cysteinyl leukotrienes (cLTs) and leukotriene B4 (LTB4). Inhibition appeared 10 min after addition of the substance and reached a maximal value after 3 h. Saiboku-To did not inhibit the release of [3H]arachidonic acid (AA) from cell membrane by calcium ionophore stimulation, or the production of cLTs and LTB4 when LTA4-free acid was used as the substrate. However, it significantly inhibited the production of cLTs and LTB4 when free AA was used as the substrate. The production of thromboxane A2 (TXA2). a cyclooxygenase metabolite, was not inhibited when AA was used as the substrate in cell free study. These results indicate that Saiboku-To selectively inhibits 5-LO activity in the metabolic pathway of AA.
柴朴汤是10种草药提取物的混合物，在日本已用于治疗支气管哮喘。研究了该药物对大鼠嗜碱性白血病细胞（RBL-1细胞）中花生四烯酸5-脂氧合酶（5-LO）代谢的抑制作用。柴朴汤显著抑制钙离子载体刺激的半胱氨酰白三烯（cLTs）和白三烯B4（LTB4）的合成。加入该物质10分钟后出现抑制作用，3小时后达到最大值。柴朴汤不抑制钙离子载体刺激引起的细胞膜中[3H]花生四烯酸（AA）的释放，也不抑制以无LTA4的酸为底物时cLTs和LTB4的产生。然而，当以游离AA为底物时，它显著抑制cLTs和LTB4的产生。在无细胞研究中以AA为底物时，环氧化酶代谢产物血栓素A2（TXA2）的产生未受抑制。这些结果表明，柴朴汤在AA代谢途径中选择性抑制5-LO活性。
