Kobayashi I, Hamasaki Y, Yamamoto S, Hayasaki R, Zaitsu M, Muro E, Matsumoto S, Ichimaru T, Miyazaki S
Saga Medical School Pediatrics, Japan.
Arerugi. 1996 Jun;45(6):577-83.
We investigated the effects of Saiboku-to, Syoseiryu-to and compornent herbs of these two Kampo-Medicines (Saiko, Hange, Bukuryo, Ogon, Koboku, Taiso, Ninjin, Kanzo, Soyo, Syokyo, Keihi, Gomisi, Saisin, Syakuyaku, Mao and Kankyo) on the production of peptide leukotrienes (LTs) and LTB4 in cultured rat basophilic leukemia (RBL)-1 cells. Cultured RBL-1 cells were stimulated with Ca ionophore, A23187, at 10(-5) M in the absence or presence of various concentrations of these substances, and the production of peptide LTs and LTB4 was measured by reversed phase-high performance liquid chromatography (RP-HPLC). The production of LTs was dose-dependently suppressed by the addition of Saibokuto. Saiboku-to (100 micrograms/ml) showed 35% and 30% inhibition on the production of peptide LTs and LTB4, respectively. These inhibitory actions of Saiboku-to on LT-synthesis were attributable to the effects of its component herbs, Ogon, Koboku and Kanzo. On the other hand, Syoseiryu-to showed no inhibitory action on LT-production. these results indicate that anti-allergic action of Saiboku-to is, at least in part, attributable to its inhibitory action on LT-synthesis.
我们研究了柴胡汤、小柴胡汤以及这两种汉方药的组成草药（柴胡、半夏、茯苓、黄芩、桂枝、大枣、人参、甘草、生姜、芍药、干姜、吴茱萸、细辛、当归、芍药、麻黄、干姜）对培养的大鼠嗜碱性白血病（RBL）-1细胞中肽白三烯（LTs）和白三烯B4（LTB4）生成的影响。在不存在或存在不同浓度这些物质的情况下，用10⁻⁵ M的钙离子载体A23187刺激培养的RBL-1细胞，并用反相高效液相色谱法（RP-HPLC）测定肽LTs和LTB4的生成。加入柴胡汤后，LTs的生成呈剂量依赖性抑制。柴胡汤（100微克/毫升）对肽LTs和LTB4的生成分别显示出35%和30%的抑制作用。柴胡汤对LT合成的这些抑制作用归因于其组成草药黄芩、桂枝和甘草的作用。另一方面，小柴胡汤对LT生成没有抑制作用。这些结果表明，柴胡汤的抗过敏作用至少部分归因于其对LT合成的抑制作用。
