Haro I, Pinto R M, Gonzalez-Dankaart J F, Perez J A, Reig F, Bosch A
Department of Peptides, CID, CSIC, Barcelona, Spain.
Microbiol Immunol. 1995;39(7):485-90. doi: 10.1111/j.1348-0421.1995.tb02232.x.
Peptide VP1 (11-25) of the capsid of hepatitis A virus was synthesized by the Fmoc-polyamide solid phase method, and administered to mice in different forms: (1) free, (2) encapsulated in multilamellar liposomes, (3) coupled to keyhole limpet hemocyanin (KHL), and (4) incorporated into a tetrameric branched lysine core. The highest anti-VP1 peptide responses were generated by synthetic peptides entrapped into liposomes and coupled to KLH. No anti-HAV response was generated with the free peptide, while all the other forms induced both anti-HAV and HAV-neutralizing antibodies. Maximum neutralization indices were observed in ascites from mice treated with liposome-entrapped and KLH peptides.
甲型肝炎病毒衣壳的肽VP1（11-25）通过Fmoc-聚酰胺固相法合成，并以不同形式给予小鼠：（1）游离形式，（2）包裹在多层脂质体中，（3）偶联到匙孔血蓝蛋白（KHL）上，以及（4）掺入四聚体分支赖氨酸核心中。包裹在脂质体中并偶联到KLH的合成肽产生了最高的抗VP1肽反应。游离肽未产生抗HAV反应，而所有其他形式均诱导了抗HAV和HAV中和抗体。在用脂质体包裹的肽和KLH肽处理的小鼠腹水中观察到最大中和指数。
