Todorović-Tirnanić M, Rolović Z, Obradović V, Bosnjaković V
Institute of Nuclear Medicine, University Clinical Center, Belgrade.
Med Pregl. 1993;46 Suppl 1:97-9.
The paper presents the new method of platelet labelling, quality control of separation and labelling, as well as the investigation of the 111In oxinate labelled platelets lifespan. Among the 23 investigated persons, there were 4 hematologically healthy persons and 19 ITP patients. Homologous and autologous labellings were done in one and 22 persons, respectively. Determination of the platelet lifespan in healthy subjects enabled the estimation of "in vitro" procedures of separation and labelling, as well as "in vivo" behaviour of the labelled platelets. Our results show that 111In oxinate labelled platelets have normal lifespan (8.9 +/- 0.6 days), which means that the procedure of separation and labelling does not damage them. In the ITP patients the platelet lifespan is shortened (0.4-4.6 days), which confirms the mechanism of thrombocytopenia. In the subject where homologous labelling had been done, we found out the existence of sensibilisation toward the donor platelets, which lead to the correction of therapy.
本文介绍了血小板标记的新方法、分离和标记的质量控制，以及111铟奥昔萘酸盐标记血小板寿命的研究。在23名被调查者中，有4名血液学健康者和19名特发性血小板减少性紫癜（ITP）患者。分别在1名和22名患者中进行了同源标记和自体标记。对健康受试者血小板寿命的测定有助于评估分离和标记的“体外”程序，以及标记血小板的“体内”行为。我们的结果表明，111铟奥昔萘酸盐标记的血小板具有正常寿命（8.9±0.6天），这意味着分离和标记程序不会损害它们。在ITP患者中，血小板寿命缩短（0.4 - 4.6天），这证实了血小板减少的机制。在进行同源标记的受试者中，我们发现了对供体血小板的致敏现象，这导致了治疗的调整。
