通过聚合酶链反应检测1型和2型单纯疱疹病毒并进行基因分型。
Detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction.
作者信息
Lucotte G, Bathelier C, Lespiaux V, Bali C, Champenois T
机构信息
International Institute of Anthropology, Paris, France.
出版信息
Mol Cell Probes. 1995 Oct;9(5):287-90. doi: 10.1016/s0890-8508(95)91508-7.
A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens.
开发了一种简单快速的聚合酶链反应（PCR）程序，用于同时检测单纯疱疹病毒1型和2型并进行分型。在同时进行的双重PCR反应中使用两对引物可以检测HSV并进行分型，其中根据琼脂糖凝胶电泳后溴化乙锭染色带确定存在的HSV类型。该PCR检测方法在约500份临床标本上进行了测试。
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