Bramono K, Tsuboi R, Ogawa H
Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
Mycoses. 1995 Sep-Oct;38(9-10):349-53. doi: 10.1111/j.1439-0507.1995.tb00063.x.
Attempts to detect inducible carbohydrate-degrading enzymes from C. albicans were performed using carbohydrate-restricted media. Although alpha-amylase (polysaccharide hydrolase) was not induced from media containing starch or dextrin, alpha-glucosidase (disaccharide hydrolase) was induced from maltose-containing medium. alpha-Glucosidase activity was detected from the intact cells in suspension and from the supernatant of both spheroplasted and mechanically broken cells, but not from the media. Enzyme activity in intact cells was pH independent and increased during logarithmic growth phase and in media containing lower concentrations of maltose. The addition of 5 mM 1-deoxynojirimycin, a competitive inhibitor of alpha-glucosidase, suppressed fungal growth by more than 50%. These results suggest that alpha-glucosidase activity is crucial for fungal growth in medium containing maltose as a sole carbon source.
利用碳水化合物限制培养基来检测白色念珠菌中可诱导的碳水化合物降解酶。虽然在含有淀粉或糊精的培养基中未诱导出α-淀粉酶（多糖水解酶），但在含麦芽糖的培养基中诱导出了α-葡萄糖苷酶（二糖水解酶）。在悬浮的完整细胞以及原生质体化细胞和机械破碎细胞的上清液中均检测到了α-葡萄糖苷酶活性，但在培养基中未检测到。完整细胞中的酶活性与pH无关，在对数生长期以及含有较低浓度麦芽糖的培养基中会增加。添加5 mM的1-脱氧野尻霉素（一种α-葡萄糖苷酶的竞争性抑制剂）可使真菌生长抑制超过50%。这些结果表明，α-葡萄糖苷酶活性对于真菌在以麦芽糖作为唯一碳源的培养基中的生长至关重要。
