Jenkins D A, Wojtacha D R, Swan P, Fleming S, Cumming A D
University Department of Medicine, Royal Infirmary of Edinburgh, UK.
Nephron. 1995;71(3):303-8. doi: 10.1159/000188736.
We studied paraffin sections of renal biopsies from 7 patients with crescentic glomerulonephritis (CGN) by in situ hybridisation, to detect sites of interleukin-2 receptor (IL-2R) mRNA expression. Frozen sections from a further patient with CGN were studied by immunohistochemistry with a monoclonal antibody to CD25, to detect IL-2R protein. Positive control sections were taken from biopsies of acute cellular renal transplant rejection and negative controls from biopsies of membranous glomerulonephritis. No autoradiographic signal was detected in negative controls. IL-2R mRNA expression was seen in rejected transplants and in sections from 4 to 7 patients with CGN. Expression was seen in cortical interstitial cells, renal tubular epithelial cells, cells within glomerular crescents and in one glomerulus at the margin of Bowman's capsule. Tubular cell expression of IL-2R protein was confirmed by immunohistochemistry. We have confirmed that IL-2R mRNA expression occurs locally within the kidneys in CGN and have identified expression in tubular epithelial cells. The results suggest that local activation of immunocompetent cells occurs in the kidney and may be of significance in the pathogenesis of CGN.
我们通过原位杂交研究了7例新月体性肾小球肾炎（CGN）患者肾活检的石蜡切片，以检测白细胞介素-2受体（IL-2R）mRNA的表达位点。用抗CD25单克隆抗体通过免疫组织化学研究了另外1例CGN患者的冰冻切片，以检测IL-2R蛋白。阳性对照切片取自急性细胞性肾移植排斥反应的活检组织，阴性对照取自膜性肾小球肾炎的活检组织。阴性对照中未检测到放射自显影信号。在移植排斥组织以及4至7例CGN患者的切片中可见IL-2R mRNA表达。在皮质间质细胞、肾小管上皮细胞、肾小球新月体内的细胞以及鲍曼囊边缘的一个肾小球中均可见表达。通过免疫组织化学证实了肾小管细胞中IL-2R蛋白的表达。我们已经证实IL-2R mRNA表达在CGN患者的肾脏局部发生，并已确定在肾小管上皮细胞中有表达。结果表明免疫活性细胞的局部激活发生在肾脏中，可能在CGN的发病机制中具有重要意义。
