Nakopoulou L, Stefanaki K, Boletis J, Papadakis J, Kostakis A, Vosnides G, Zeis P M
Department of Pathology, University of Athens, Medical School, Greece.
Nephrol Dial Transplant. 1994;9(7):764-9.
Epidermal growth factor (EGF), a polypeptide with a potent mitogen activity, and its receptor [EGFR] have been previously identified in the kidney, but their expression in normal and diseased kidneys has not been fully elucidated. In order to evaluate EGFR in various histological types of renal injury, EGFR expression was studied by the immunohistochemical avidin-biotin complex (ABC) method with a monoclonal antibody EGFR1 on paraffin sections from 10 normal kidneys, 56 renal biopsies with various types of glomerulonephritis (GN), and 20 renal grafts with rejection. EGFR expression was observed in (a) 3 of 10 (30%) normal kidneys, (b) 17 of 39 (43.6%) renal biopsies with various types of GN mainly in membranous GN (57%) and in focal segmental glomerulosclerosis (FSG) (62.5%), (c) 6 of 17 (35.3%) biopsies with various types of systemic lupus erythematosus GN, and (d) 12 of 20 (60%) renal grafts with acute (42.9%) and chronic (69.2%) rejection. EGFR was mainly localized to the epithelial cells of the distal and collecting tubules and extraglomelar vessels, while it was observed less frequently in parietal epithelial cells and along glomerular basement membranes. Notably EGFR was detected in the epithelial cells adjacent to adhesions with Bowman's capsule and in the connective tissue of fibrocellular crescents. In conclusion, EGFR expression was observed more frequently in diseased than in normal kidneys. The increased incidence of EGFR expression in FSG, in chronic rejection, in small adhesions with Bowman's capsule and fibrocellular crescents suggest that EGF/EGFR may be correlated with a disturbed extracellular matrix production resulting in formation of early sclerotic lesions.
表皮生长因子（EGF）是一种具有强大促有丝分裂活性的多肽，其受体[EGFR]先前已在肾脏中被鉴定出来，但它们在正常和患病肾脏中的表达尚未完全阐明。为了评估不同组织学类型肾损伤中的EGFR，采用免疫组织化学抗生物素蛋白-生物素复合物（ABC）法，用单克隆抗体EGFR1对10个正常肾脏、56例各种类型肾小球肾炎（GN）的肾活检组织以及20例发生排斥反应的肾移植组织的石蜡切片进行EGFR表达研究。EGFR表达见于：（a）10个正常肾脏中的3个（30%）；（b）39例各种类型GN肾活检组织中的17个（43.6%），主要见于膜性GN（57%）和局灶节段性肾小球硬化（FSGS）（62.5%）；（c）17例各种类型系统性红斑狼疮性GN活检组织中的6个（35.3%）；以及（d）20例发生急性（42.9%）和慢性（69.2%）排斥反应的肾移植组织中的12个（60%）。EGFR主要定位于远端小管和集合小管的上皮细胞以及球外血管，而在壁层上皮细胞和沿肾小球基底膜中较少见到。值得注意的是，在与鲍曼囊粘连相邻的上皮细胞以及纤维细胞性新月体的结缔组织中检测到了EGFR。总之，EGFR表达在患病肾脏中比在正常肾脏中更常见。FSGS、慢性排斥反应、与鲍曼囊的小粘连以及纤维细胞性新月体中EGFR表达发生率的增加表明，EGF/EGFR可能与细胞外基质产生紊乱相关，导致早期硬化性病变的形成。
