Aoki Y, Akagi K, Kitada N, Tanaka Y, Fritz-Zieroth B
Department of Radiology, Kansai Medical University.
Nihon Igaku Hoshasen Gakkai Zasshi. 1995 Nov;55(14):987-92.
We analyzed tumor growth delay in experimental tumors after hyperthermia alone, hydralazine (HDZ) injection alone and the combination of these modalities. We also analyzed the energy parameter (ATP/Pi ratio) obtained by 31P-MRS (magnetic resonance spectroscopy). The purpose of this study was to evaluate the usefulness of 31P-MRS as an index of anti-tumor effect. FM3A tumor cells were transplanted subcutaneously in the hind legs of C3H/He mice. We dipped the tumors into a heated circulating water bath. 31P-MRS was performed with a CSI spectrometer. The anti-tumor effect obtained with HDZ alone was insignificant, but combined treatment with hyperthermia and HDZ had a significant synergistic effect. The ATP/Pi ratios for all groups treated separately with HDZ or hyperthermia were not different from the control, but the ATP/Pi ratio decreased after combined use of these agents. There was a significant correlation between the decrease in ATP/Pi ratio and tumor growth delay. We observed a direct relation between the delay in tumor growth and the decline in ATP/Pi ratio after combined treatment with HDZ and hyperthermia. The ATP/Pi ratio 24 hr after treatment may be useful in predicting the efficacy of the combined use of HDZ and hyperthermia.
我们分析了单纯热疗、单独注射肼屈嗪（HDZ）以及这两种方式联合应用后实验性肿瘤的生长延迟情况。我们还分析了通过31P磁共振波谱（MRS）获得的能量参数（ATP/无机磷酸比值）。本研究的目的是评估31P-MRS作为抗肿瘤效果指标的实用性。将FM3A肿瘤细胞皮下移植到C3H/He小鼠的后腿。我们把肿瘤浸入加热的循环水浴中。用化学位移成像（CSI）光谱仪进行31P-MRS检测。单独使用HDZ获得的抗肿瘤效果不显著，但热疗与HDZ联合治疗具有显著的协同效应。单独用HDZ或热疗治疗的所有组的ATP/无机磷酸比值与对照组无差异，但联合使用这些药物后ATP/无机磷酸比值降低。ATP/无机磷酸比值的降低与肿瘤生长延迟之间存在显著相关性。我们观察到HDZ与热疗联合治疗后肿瘤生长延迟与ATP/无机磷酸比值下降之间存在直接关系。治疗后24小时的ATP/无机磷酸比值可能有助于预测HDZ与热疗联合使用的疗效。
