Eskitürk A, Söyletir G, Peker O, Haklar G, Yalçin A S, Demirel M, Işgör A
Department of Microbiology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
Res Exp Med (Berl). 1995;195(3):163-9. doi: 10.1007/BF02576785.
Male Wistar albino rats were allocated to three groups-sham operated (n: 10), splenectomized (n: 20) and splenic autotransplanted (n: 10). Twelve weeks after operation, all were challenged with 1.8 x 10(8) cfu/ml Pseudomonas aeruginosa intranasally. Half of the splenectomized rats received imipenem-cilastatin after 2 h of bacterial challenge. Mortality was then observed for the next 12 days. All animals were autopsied and liver, kidney, spleen and lung specimens were processed for microbiological culture and histopathological examination. In 80% of autotransplanted rats, splenic tissue regeneration was histopathologically verified. Hemoglobin oxidation of erythrocytes increased in splenectomized rats and remained close to control levels in the autotransplanted group. No significant difference was detected between IgM levels of splenectomized and autotransplanted groups. Mortality rates were the same for all groups, except that splenectomized animals given antimicrobial therapy had increased survival rates. In conclusion, it is likely that the spleen has no role in protection against pulmonary sepsis and that only appropriate antimicrobial therapy can protect the splenectomized host from Pseudomonas sepsis.
雄性Wistar白化大鼠被分为三组：假手术组（n = 10）、脾切除组（n = 20）和脾自体移植组（n = 10）。术后12周，所有大鼠均经鼻内接种1.8×10⁸ cfu/ml铜绿假单胞菌进行激发。脾切除组的半数大鼠在细菌激发2小时后接受亚胺培南-西司他丁治疗。随后观察接下来12天的死亡率。所有动物均进行尸检，并对肝脏、肾脏、脾脏和肺组织标本进行微生物培养和组织病理学检查。在80%的自体移植大鼠中，组织病理学证实了脾组织再生。脾切除大鼠红细胞的血红蛋白氧化增加，而自体移植组则接近对照水平。脾切除组和自体移植组的IgM水平未检测到显著差异。除接受抗菌治疗的脾切除动物存活率增加外，所有组的死亡率相同。总之，脾脏可能在预防肺部脓毒症方面不起作用，只有适当的抗菌治疗才能保护脾切除宿主免受铜绿假单胞菌脓毒症的侵害。
