Hendrich C, Sudmann J, Mommsen J, Siebert W E
Orthopädische Universitätsklinik, Würzburg.
Z Orthop Ihre Grenzgeb. 1995 Nov-Dec;133(6):520-3. doi: 10.1055/s-2008-1039932.
Since ten years different laser systems have been used in arthroscopic procedures and percutaneous laser disc decompression. A potential mutagenic risk has not been excluded yet. From photobiology it is known that light in the UV-range is the main source of mutations. Therefore the aim of our study was to determine the mutagenic risk of laser systems at UV-wavelengths. The BALB/3T3 cell line was irradiated at the Excimer wavelengths 248 nm and 308 nm. Transformation rates were compared with non-irradiated cells and the effect of 1 Gy-Roentgen irradiation. Pulse energy, frequency and irradiation time were varied over a broad range. The effect of double irradiation was also examined. For 308 nm no significant transformation rate was found (p < 0.18). In contrast 248 nm showed a highly significant transformation rate (p < 10(-9). Transformation rate after Roentgen irradiation exceeded that of the negative control about two orders. While a certain mutagenic risk exists for 248 nm application of the wavelength 308 nm seems harmless. Our data therefore represent an essential contribution to long term laser safety both for the physician and his patient.
十年来，不同的激光系统已被用于关节镜手术和经皮激光椎间盘减压术。潜在的诱变风险尚未排除。从光生物学可知，紫外线范围内的光是突变的主要来源。因此，我们研究的目的是确定激光系统在紫外线波长下的诱变风险。用准分子激光波长248纳米和308纳米照射BALB/3T3细胞系。将转化率与未照射细胞以及1戈瑞伦琴照射的效果进行比较。脉冲能量、频率和照射时间在很宽的范围内变化。还研究了双重照射的效果。对于308纳米，未发现显著的转化率（p < 0.18）。相比之下，248纳米显示出高度显著的转化率（p < 10^(-9)）。伦琴照射后的转化率比阴性对照高出约两个数量级。虽然248纳米的应用存在一定的诱变风险，但308纳米波长似乎无害。因此，我们的数据对医生及其患者的长期激光安全具有重要贡献。
