Trentacoste J, Thompson K, Parrish R K, Hajek A, Berman M R, Ganjei P
Ophthalmology. 1987 Feb;94(2):125-9. doi: 10.1016/s0161-6420(87)33487-6.
In response to concerns regarding possible DNA damage by far ultraviolet radiation during excimer laser corneal surgery, the mutagenic potential of an argon fluoride excimer laser (193 nm) on BALB/3T3 mouse fibroblasts grown in tissue culture was investigated. The cumulative incidence of anaplastic transformation after subablative radiant exposures from 3.5 mJ/cm2/pulse to 13.4 mJ/cm2/pulse was 3.6% of all cell cultures. The incidence of anaplastic transformation in nonirradiated controls was 4.2%. Transformation after exposure to x-ray radiation (60.9 rad) was 98.8%. The difference between the incidence of transformation of nonirradiated controls or excimer-treated cultures compared with x-ray radiation-treated cells was significant P less than 0.0001 (chi square test). In this standard cell line, 193-nm laser energy does not appear to have substantial mutagenic potential.
针对准分子激光角膜手术中极紫外辐射可能造成DNA损伤的担忧，研究了氟化氩准分子激光（193纳米）对组织培养中生长的BALB/3T3小鼠成纤维细胞的致突变潜力。从3.5毫焦/平方厘米/脉冲到13.4毫焦/平方厘米/脉冲进行亚消融辐射暴露后，间变转化的累积发生率为所有细胞培养物的3.6%。未辐照对照组中间变转化的发生率为4.2%。暴露于X射线辐射（60.9拉德）后的转化率为98.8%。与X射线辐射处理的细胞相比，未辐照对照组或准分子激光处理培养物的转化发生率差异显著（P<0.0001，卡方检验）。在这种标准细胞系中，193纳米激光能量似乎没有实质性的致突变潜力。
