Welling D B, Daniels R L, Brainard J, Western L M, Prior T W
Department of Otolaryngology, Ohio State University, College of Medicine, Columbus 43210, USA.
Am J Otol. 1994 Sep;15(5):639-43.
Neurotropic viruses have been postulated to play a role in the development of Menière's disease (MD). The purpose of this study was to evaluate the endolymphatic sacs of patients undergoing surgery for MD in a single-blind study for evidence of herpes simplex virus (HSV), varicella zoster (VZ), or cytomegalovirus (CMV) DNA. Polymerase chain reaction (PCR) was used as the method of detection because of its sensitivity, specificity, and applicability to fresh, as well as fixed tissues. Twenty-two patients with MD and 11 control patients with vestibular schwannomas had a portion of the endolymphatic sac removed at the time of surgery. The specimens were then evaluated for herpes simplex type and 2, varicella zoster, and cytomegalovirus DNA. Herpes simplex virus DNA was detected in 2 of the 22 extracts from the endolymphatic sacs obtained from patients with MD. There was no evidence of a positive signal obtained with any of the other viral DNA probes when PCR was performed on the control tissue extracts or the other MD tissue extracts. These results do not demonstrate a significant difference and do not statistically support the postulate that ongoing viral infection in the endolymphatic sac is a frequent factor in the development of Menière's disease.
嗜神经病毒被认为在梅尼埃病（MD）的发病过程中起作用。本研究的目的是在一项单盲研究中，评估接受MD手术患者的内淋巴囊，以寻找单纯疱疹病毒（HSV）、水痘带状疱疹病毒（VZ）或巨细胞病毒（CMV）DNA的证据。由于聚合酶链反应（PCR）具有敏感性、特异性且适用于新鲜组织和固定组织，因此被用作检测方法。22例MD患者和11例前庭神经鞘瘤对照患者在手术时切除了部分内淋巴囊。然后对标本进行单纯疱疹病毒1型和2型、水痘带状疱疹病毒及巨细胞病毒DNA检测。在从MD患者获得的22份内淋巴囊提取物中，有2份检测到单纯疱疹病毒DNA。对对照组织提取物或其他MD组织提取物进行PCR时，没有证据表明使用任何其他病毒DNA探针能获得阳性信号。这些结果没有显示出显著差异，也没有统计学上的证据支持内淋巴囊持续病毒感染是梅尼埃病发病常见因素这一假设。
