Giarnieri E, Alderisio M, Valli C, Vecchione A, Forte A, Turano R, Pulcini M
Dipartimento di Medicina Sperimentale e Patologia, Università La Sapienza, Roma, Italy.
Anticancer Res. 1995 Sep-Oct;15(5B):2049-53.
The nm23 gene was originally identified by differential hybridization between two murine melanoma cell sublines with low and high metastatic potential. Nm23 is localized on chromosome 17q21.3-22. Allelic deletions of chromosome 17 have been related to the progression of colorectal carcinomas. We have evaluated and compared the expression of nm23 NPD kinase protein using an immunohistochemical method and DNA ploidy evaluation with image analysis. This study was performed on 20 patients, who underwent surgery for colorectal carcinoma. Patients were followed up during the period from 1992 to 1994. Results have shown an association between the parameters obtained for the nm23 NPD kinase protein expression, and aneuploid DNA and neoplastic progression. The expression of nucleoside diphosphate (NDP) kinase mm23 has been reported to be inversely related to the metastatic potential of experimental cells in human breast cancer. A relationship between the positivity in protein expression of gene product in the allele nm23 H1 and the state of the lymph nodes has also been found.
nm23基因最初是通过对具有低转移潜能和高转移潜能的两个小鼠黑色素瘤细胞亚系进行差异杂交而鉴定出来的。Nm23定位于17号染色体的q21.3 - 22区域。17号染色体的等位基因缺失与结直肠癌的进展有关。我们使用免疫组织化学方法和图像分析进行DNA倍体评估，对nm23 NPD激酶蛋白的表达进行了评估和比较。本研究对20例行结直肠癌手术的患者进行。在1992年至1994年期间对患者进行了随访。结果显示，nm23 NPD激酶蛋白表达所获得的参数与非整倍体DNA及肿瘤进展之间存在关联。据报道，核苷二磷酸（NDP）激酶mm23的表达与人类乳腺癌实验细胞的转移潜能呈负相关。在等位基因nm23 H1中基因产物的蛋白表达阳性与淋巴结状态之间也发现了一种关系。
