Alderisio M, Cenci M, Valli C, Russo A, Bazan V, Dardanoni G, Cucciarre S, Carreca I, Macaluso M P, Tomasino R M, Vecchione A
Department of Experimental Medicine and Pathology, University La Sapienza, Roma, Italy.
Anticancer Res. 1998 Nov-Dec;18(6A):4225-30.
To explain the overall survival (OS) and disease free survival (DFS) in relation to nm23-H1 protein, DNA-ploidy and S-phase fraction (SPF) in transitional cell carcinoma of the bladder.
Ninety-four samples were obtained from patients with transitional cell carcinoma of the bladder examined between 1994 and 1996. The patients were underwent cistectomy or surgical biopsy and the material was histologically evaluated according to World Health Organization classification. Nm23-H1 protein expression in immunohistological staining and DNA ploidy, S-phase fraction by flow cytometric were performed.
The correlation between OS and staging, grading, DNA-ploidy and S-phase was significant; whereas the overall survival and nm23-H1 protein, was not significant. The relationship between DFS and stage, DNA-ploidy and S-phase had a significant value. The correlation between DFS and age, sex, grading and nm23-H1 protein was not significant. There was no significant difference in age, sex, stage, grading, DNA-ploidy and SPF distribution between patients with nm23-H1 positive bladder cancer and those with nm23-H1 negative tumours.
In our study, multivariate analysis showed that stage, ploidy and SPF were the strongest prognostic factors in predicting disease-free survival and prolonged survival, while nm23-H1 expression was not related to disease progression and/or prolonged survival. This expression, therefore, does not appear to be an independent prognostic factor in bladder cancer, although a still larger number of patients and a longer follow-up period are now needed for a definitive assessment of the prognostic significance of nm23-H1 expression.
探讨膀胱移行细胞癌中nm23-H1蛋白、DNA倍体和S期分数（SPF）与总生存期（OS）和无病生存期（DFS）的关系。
1994年至1996年间对94例膀胱移行细胞癌患者的样本进行了研究。患者接受了膀胱切除术或手术活检，并根据世界卫生组织分类对材料进行了组织学评估。进行了免疫组织化学染色检测nm23-H1蛋白表达以及通过流式细胞术检测DNA倍体和S期分数。
OS与分期、分级、DNA倍体和S期之间存在显著相关性；而总生存期与nm23-H1蛋白之间无显著相关性。DFS与分期、DNA倍体和S期之间的关系具有显著意义。DFS与年龄、性别、分级和nm23-H1蛋白之间的相关性不显著。nm23-H1阳性膀胱癌患者与nm23-H1阴性肿瘤患者在年龄、性别、分期、分级、DNA倍体和SPF分布方面无显著差异。
在我们的研究中，多因素分析表明分期、倍体和SPF是预测无病生存期和延长生存期的最强预后因素，而nm23-H1表达与疾病进展和/或延长生存期无关。因此，尽管现在需要更多的患者和更长的随访期来明确评估nm23-H1表达的预后意义，但这种表达似乎不是膀胱癌的独立预后因素。
