Kohlberger P, Kainz C, Kölbl H, Czerwenka K, Breitenecker G
Department of Gynecology & Obstetrics, Vienna University Medical School, Austria.
Anticancer Res. 1995 Sep-Oct;15(5B):2307-11.
Basaloid carcinomas of the vulva have been reported to occur mostly in young women and are often associated with Human Papillomavirus (HPV) infection, whereas older women mostly suffer from keratinizing squamous cell carcinomas unassociated with HPV. The rare case of a 75 year old woman coincidentally suffering from a basaloid carcinoma and a keratinizing squamous cell carcinoma of the vulva is reported. With in situ hybridisation, hybrid capture and immunohistochemical methods we searched for HPV and expression of cytokeratin AE1/AE3, cytokeratin HMW and cytokeratin 8, 18, 19. HPV was not found in either of the carcinomas. Cytokeratin HMW showed a focal staining reaction in the keratinizing squamous cell carcinoma and no expression in the basaloid carcinoma. Immunohistochemical staining reaction for cytokeratin AE1/AE3 was found in both tumors with the same staining pattern. Cytokeratin 8, 18, 19 was not detected in either of the two carcinomas.
据报道，外阴基底样癌大多发生于年轻女性，且常与人乳头瘤病毒（HPV）感染相关，而老年女性大多患与HPV无关的角化性鳞状细胞癌。本文报告了1例75岁老年女性同时患外阴基底样癌和角化性鳞状细胞癌的罕见病例。我们采用原位杂交、杂交捕获和免疫组化方法检测HPV以及细胞角蛋白AE1/AE3、高分子量细胞角蛋白和细胞角蛋白8、18、19的表达。在两种癌中均未发现HPV。高分子量细胞角蛋白在角化性鳞状细胞癌中呈局灶性染色反应，在基底样癌中无表达。细胞角蛋白AE1/AE3在两种肿瘤中的免疫组化染色反应相同。在两种癌中均未检测到细胞角蛋白8、18、19。
