培养过程中脂多糖B对人胎儿淋巴细胞的刺激作用。

Stimulation of human fetal lymphocytes by lipopolysaccharide B in culture.

作者信息

Turunen O, Lundqvist C, de la Chapelle A

出版信息

Scand J Immunol. 1977;6(4):335-42. doi: 10.1111/j.1365-3083.1977.tb00402.x.

DOI:10.1111/j.1365-3083.1977.tb00402.x

Abstract

Cultures of Isopaque-Ficoll-isolated lymphocytes from three human sources were compared with respect to the effect of mitogens. The cell sources were maternal blood immediately after delivery, cord blood, and blood obtained by heart puncture of 10-20-week aborted fetuses. Lipopolysaccharide B (LPS) induced incorporation of tritiated thymidine, blastic transformation, and mitotic activity in cord and fetal, but not maternal, cells. The stimulation reached a maximum on days 4-8 of culture. It was stronger than the spontaneous transformation often displayed by fetal cells. If fetal cells spontaneously occurring in the blood of pregnant women were to react in a similar way, it should be possible to selectively stimulate the fetal cells with LPS. Such transformed fetal cells could then be isolated from cultures of maternal blood samples and used for antenatal diagnosis of fetal disease.

摘要

就促细胞分裂剂的作用而言，对来自三种人类来源的异泛影葡胺-菲可分离淋巴细胞培养物进行了比较。细胞来源为分娩后立即采集的母血、脐带血以及通过对10-20周流产胎儿进行心脏穿刺获得的血液。脂多糖B（LPS）可诱导脐带血和胎儿血中的细胞掺入氚标记的胸腺嘧啶核苷、发生细胞母细胞转化以及有丝分裂活性，但对母血中的细胞无此作用。刺激作用在培养的第4-8天达到最大值。其作用强于胎儿细胞常表现出的自发转化。如果孕妇血液中自然存在的胎儿细胞以类似方式作出反应，那么就有可能用LPS选择性地刺激胎儿细胞。然后可以从母血样本培养物中分离出这种转化的胎儿细胞，并用于胎儿疾病的产前诊断。