Iwata M, Matuhasi T
Jpn J Exp Med. 1978 Oct;48(5):401-10.
The antigencity of CS-1170, a newly developed semi-synthetic cephamycin, and it's cross-reactivity with beta-lactam antibiotics were studied. The antigencity was confirmed by the following tests: 1)the passive hemagglutination test with anti-CS-1170 antisera of guinea pigs immunized with CS-1170 plus Freund complete adjuvant, 2)lymphocyte proliferation response in vitro stimulated with this drug in the guinea pigs, 3) the PCA test using mice immunized with CS-1170-protein conjugates. The antibody to CS-1170 appears to be directed to the acyl side chain because cyanomethylthioacetylglycine, an univalent acyl side chain of the CS-1170, can significantly cross-react with the antibody. A methoxy group on the C-7 alpha-position of the antibiotic plays no important part for the antigenic specificity of the molecule. The cross-reactivity of CS-1170 with cefazolin, cephalothin, benzylpenicillin and ampicillin was only to a minimal extent in the passive hemagglutination test. The cross-reactivity of CS-1170 and the related antibiotics was not observed among them in the PCA system with IgE anti-CS-1170. These findings support a conclusion that CS-1170 is one of beta-lactam antibiotic with an immunologically minimal cross-reactivity to the related antibiotics.
对新开发的半合成头孢霉素CS - 1170的抗原性及其与β-内酰胺类抗生素的交叉反应性进行了研究。通过以下试验证实了其抗原性：1）用CS - 1170加弗氏完全佐剂免疫的豚鼠的抗CS - 1170抗血清进行的被动血凝试验；2）该药物在豚鼠体内刺激的体外淋巴细胞增殖反应；3）用CS - 1170 - 蛋白质偶联物免疫的小鼠进行的PCA试验。针对CS - 1170的抗体似乎针对酰基侧链，因为CS - 1170的单价酰基侧链氰甲基硫代乙酰甘氨酸可与该抗体发生显著交叉反应。抗生素C - 7α位上的甲氧基对分子的抗原特异性不起重要作用。在被动血凝试验中，CS - 1170与头孢唑林、头孢噻吩、苄青霉素和氨苄青霉素的交叉反应仅在极小程度上存在。在使用抗CS - 1170 IgE的PCA系统中，未观察到CS - 1170与相关抗生素之间的交叉反应。这些发现支持了一个结论，即CS - 1170是一种与相关抗生素免疫交叉反应极小的β-内酰胺类抗生素。
