Buseyne F, Février M, Garcia S, Gougeon M L, Rivière Y
Unité de Virologie et Immunologie Cellulaire, URA CNRS 1157, Institut Pasteur, Paris, France.
Virology. 1996 Nov 1;225(1):248-53. doi: 10.1006/viro.1996.0597.
CD8+ T cells may play a beneficial role in human immunodeficiency virus (HIV)-infected patients by two mechanisms. HIV-specific cytotoxic activity and secretion of a soluble mediator(s) that inhibits HIV replication in vitro. Here we characterized both activities mediated by an HIV p24gag-specific cytotoxic T lymphocyte (CTL) CD8+ clone derived from an HIV-infected patient. When the CTL clone was mixed with HIV-infected autologous CD4+ T cells, viral replication was suppressed. This viral inhibition was observed in heterologous CD4+ T cells and when CD8+ and CD4+ populations were separated by a semipermeable membrane, demonstrating the involvement of a diffusible factor(s). The lysis of autologous HIV-infected T cells was also detected. However, HIV suppression was more efficient when CD4+ and CD8+ T cells shared major histocompatibility complex alleles and were in direct contact. Thus, one and the same CD8+ T cell population can mediate both lysis of HIV-infected targets and nonlytic suppression of HIV replication. These results underline the multiple roles of CD8+ T lymphocytes in the suppression of HIV-infected cells.
CD8 + T细胞可能通过两种机制在人类免疫缺陷病毒（HIV）感染患者中发挥有益作用。一是具有HIV特异性细胞毒性活性，二是分泌一种可在体外抑制HIV复制的可溶性介质。在此，我们对源自一名HIV感染患者的HIV p24gag特异性细胞毒性T淋巴细胞（CTL）CD8 +克隆介导的这两种活性进行了表征。当CTL克隆与HIV感染的自体CD4 + T细胞混合时，病毒复制受到抑制。在异源CD4 + T细胞中以及当CD8 +和CD4 +群体通过半透膜分离时也观察到了这种病毒抑制作用，这表明存在一种可扩散因子。还检测到了自体HIV感染T细胞的裂解。然而，当CD4 +和CD8 + T细胞共享主要组织相容性复合体等位基因并直接接触时，HIV抑制更为有效。因此，同一个CD8 + T细胞群体可以介导HIV感染靶标的裂解以及HIV复制的非裂解性抑制。这些结果强调了CD8 + T淋巴细胞在抑制HIV感染细胞中的多重作用。
