Goplerud J M, Kim S, Delivoria-Papadopoulos M
Department of Pediatrics, Hospital of the University of Pennsylvania, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Brain Res. 1995 Oct 23;696(1-2):161-4. doi: 10.1016/0006-8993(95)00861-j.
To compare the effect of 21% vs. 100% oxygen during post-asphyxial reoxygenation on brain cell membrane function in the striatum, 20 anesthetized, ventilated newborn piglets were studied: group 1 (normoxia, n = 5), group 2 (asphyxia, no reoxygenation, n = 5), group 3 (asphyxia followed by reoxygenation with 21% O2, n = 5), and group 4 (asphyxia followed by reoxygenation with 100% O2, n = 5). Asphyxia was induced by a stepwise reduction in FiO2 at 20 min intervals from 21% to 14%, 11%, and 8%. Following a total 60 min of asphyxia, piglets in groups 3 and 4 were recovered for 2 h with either 21% or 100% O2. Na+,K(+)-ATPase activity (mumol Pi/mg protein/h) in striatal cell membranes was 31 +/- 1, 22 +/- 2, 32 +/- 2 and 26 +/- 1 in groups 1, 2, 3 and 4, respectively. Na+,K(+)-ATPase activities in groups 2 and 4 were significantly lower than in groups 1 and 3 (p < 0.01). Piglets recovered post-asphyxia for 2 h with 21% O2 had restoration of Na+,K(+)-ATPase activity to baseline levels, while those treated with 100% O2 during recovery had persistent Na+,K(+)-ATPase inhibition of 16%. This could result from increased free radical production during reoxygenation with 100% O2 which could contribute to post-asphyxial cellular injury in the striatum.
为比较窒息后复氧期间21%与100%氧气对纹状体中脑细胞膜功能的影响，对20只麻醉、通气的新生仔猪进行了研究：第1组（常氧，n = 5），第2组（窒息，不复氧，n = 5），第3组（窒息后用21%氧气复氧，n = 5），第4组（窒息后用100%氧气复氧，n = 5）。通过每隔20分钟将吸入氧浓度从21%逐步降至14%、11%和8%来诱导窒息。在总共60分钟的窒息后，第3组和第4组的仔猪分别用21%或100%氧气复苏2小时。纹状体细胞膜中Na +,K(+)-ATP酶活性（μmol Pi/mg蛋白质/小时）在第1、2、3和4组中分别为31±1、22±2、32±2和26±1。第2组和第4组中的Na +,K(+)-ATP酶活性显著低于第1组和第3组（p < 0.01）。窒息后用21%氧气复苏2小时的仔猪，其Na +,K(+)-ATP酶活性恢复到基线水平，而复苏期间用100%氧气治疗的仔猪，其Na +,K(+)-ATP酶持续抑制16%。这可能是由于用100%氧气复氧期间自由基产生增加，这可能导致纹状体中窒息后细胞损伤。
