一种用于白细胞的新型99mTc标记方法：与99mTc-HMPAO的体外和体内比较。

Welling M, Feitsma H I, Blok D, Calame W, Ensing G J, Goedemans W, Pauwels E K

Department of Diagnostic Radiology and Nuclear Medicine, University Hospital, Leiden, The Netherlands.

Q J Nucl Med. 1995 Jun;39(2):89-98.

A new method for the labelling of mixed leucocytes with 99mTc-tropolone was optimized and compared with a 99mTc-HMPAO leucocyte labelling procedure in vitro and in vivo. In the present study, leucocytes obtained from patients suffering from Crohn's disease, were isolated and labelled with 99mTc-HMPAO or labelled according the new 99mTc-tropolone procedure using 9.8 mM tropolone, 1 microM stannous chloride and 0.8 mM potassium borohydride (KBH4) at pH 5.5-6. Labelling efficiency with 99mTc-tropolone yielded 92 +/- 3%, which is higher compared to the 99mTc-HMPAO labelling procedure (64 +/- 13%) using 10(8) of leucocytes. In vitro stability and viability of both the tropolone and the HMPAO labelled cells was investigated. The viability test of the 99mTc-labelled leucocytes was performed in autologous plasma at 37 degrees C and compared with unlabelled leucocytes. After 18 hours of incubation a significant (P < 0.05) higher stability was observed for 99mTc-tropolone labelled leucocytes (84 +/- 5%) compared with that of 99mTc-HMPAO labelled leucocytes (73 +/- 5%). The viability of the 99mTc-labelled leucocytes observed for both labelling procedures was similar to unlabelled leucocytes. In vivo experiments were performed in mice. 99mTc-tropolone or 99mTc-HMPAO labelled murine mixed leucocytes were injected in mice, with a Staphylococcus aureus ATCC 25923 thigh infection. Analysis of scintigraphic images yielded a faster clearance of the 99mTc-tropolone labelled leucocytes. This was most likely due to a significant (P < 0.02) higher liver uptake at 4 hours after administration of the 99mTc-tropolone labelled leucocytes (19%) in comparison with 99mTc-HMPAO labelled cells (9%). Faster and significant (P < 0.02) higher accumulation of the 99mTc-tropolone labelled leucocytes was observed at the site of infection compared with 99mTc-HMPAO labelled leucocytes at all time-intervals after the administration of the 99mTc-labelled leucocytes. The new 99mTc-tropolone leucocyte labelling procedure, offers an attractive low-cost agent for research purposes.

一种用99mTc - 托酚酮标记混合白细胞的新方法得到了优化，并在体外和体内与99mTc - HMPAO白细胞标记程序进行了比较。在本研究中，从克罗恩病患者身上获取白细胞，分离后用99mTc - HMPAO标记，或按照新的99mTc - 托酚酮程序标记，该程序使用9.8 mM托酚酮、1 microM氯化亚锡和0.8 mM硼氢化钾（KBH4），pH值为5.5 - 6。用99mTc - 托酚酮标记的效率为92±3%，与使用10^8个白细胞的99mTc - HMPAO标记程序（64±13%）相比更高。研究了托酚酮和HMPAO标记细胞的体外稳定性和活力。99mTc标记白细胞的活力测试在37℃的自体血浆中进行，并与未标记的白细胞进行比较。孵育18小时后，观察到99mTc - 托酚酮标记的白细胞（84±5%）比99mTc - HMPAO标记的白细胞（73±5%）具有显著更高（P < 0.05）的稳定性。两种标记程序观察到的99mTc标记白细胞的活力与未标记的白细胞相似。在小鼠身上进行了体内实验。将99mTc - 托酚酮或99mTc - HMPAO标记的小鼠混合白细胞注射到患有金黄色葡萄球菌ATCC 25923大腿感染的小鼠体内。闪烁图像分析显示99mTc - 托酚酮标记的白细胞清除更快。这很可能是由于在注射99mTc - 托酚酮标记的白细胞后4小时，肝脏摄取显著更高（P < 0.02）（19%），而99mTc - HMPAO标记的细胞为9%。与99mTc - HMPAO标记的白细胞相比，在注射99mTc标记的白细胞后的所有时间间隔内，99mTc - 托酚酮标记的白细胞在感染部位的积累更快且显著更高（P < 0.02）。新的99mTc - 托酚酮白细胞标记程序为研究目的提供了一种有吸引力的低成本试剂。