锝-99m d，l-六甲基丙二胺肟和锝-99m d，l-环丙胺肟作为白细胞标记剂的比较研究。

A comparative study of 99Tcm-d,l-HMPAO and 99Tcm-d,l-CBPAO as leukocyte-labelling agents.

作者信息

Tsai C S, Kao C H, Yeh S J, Wang S J

机构信息

Institute of Nuclear Medicine, National Tsing Hua University, Hsin Chu, Taiwan, Republic of China.

出版信息

Nucl Med Commun. 1996 Jan;17(1):76-9. doi: 10.1097/00006231-199601000-00013.

DOI:10.1097/00006231-199601000-00013

PMID:8692477

Abstract

An attempt was made to use 99Tcm-d,l-cyclobutylpropylene amine oxime (99Tcm-d,l-CBPAO) to label leukocytes. The radiochemical purity of 99Tcm-d,l-CBPAO, labelling efficiency of leukocytes, cell viability of labelled leukocytes and stability of 99Tcm-d,l-CBPAO-labelled leukocytes were calculated. In comparison with the commercial cell-labelling agent 99Tcm-d,l-hexamethylpropylene amine oxime (99Tcm-d,l-HMPAO), (1) the radiochemical purity of 99Tcm-d,l-CBPAO was higher than that of 99Tcm-d,l-HMPAO immediately after and 1, 2, 4, 6, 8 and 24 h after 99Tcm labelling; (2) the labelling efficiency of 99Tcm-d,l-CBPAO-labelled leukocytes was lower than that of 99Tcm-d,l-HMPAO; (3) the viability of labelled white blood cells was high for both agents; and (4) the stability of 99Tcm-d,l-CBPAO-labelled leukocytes was better than that of 99Tcm-d,l-HMPAO after 1, 2, 4, 6, 8 and 24 h. In conclusion, although the labelling efficiency of 99Tcm-d,l-CBPAO is lower, it is more stable than 99Tcm-d,l-HMPAO and provides greater stability of 99Tcm-d,l-CBPAO-labelled leukocytes. Therefore, 99Tcm-d,l-CBPAO has the potential to replace 99Tcm-d,l-HMPAO as a commercial leukocyte-labelling agent.

摘要

尝试使用99锝-d，l-环丁基丙二胺肟（99Tcm-d，l-CBPAO）标记白细胞。计算了99Tcm-d，l-CBPAO的放射化学纯度、白细胞标记效率、标记白细胞的细胞活力以及99Tcm-d，l-CBPAO标记白细胞的稳定性。与市售细胞标记剂99锝-d，l-六甲基丙二胺肟（99Tcm-d，l-HMPAO）相比，（1）99Tcm标记后即刻及1、2、4、6、8和24小时，99Tcm-d，l-CBPAO的放射化学纯度高于99Tcm-d，l-HMPAO；（2）99Tcm-d，l-CBPAO标记白细胞的效率低于99Tcm-d，l-HMPAO；（3）两种试剂标记的白细胞活力均较高；（4）99Tcm-d，l-CBPAO标记白细胞在1、2、4、6、8和24小时后的稳定性优于99Tcm-d，l-HMPAO。总之，尽管99Tcm-d，l-CBPAO的标记效率较低，但其比99Tcm-d，l-HMPAO更稳定，且99Tcm-d，l-CBPAO标记白细胞的稳定性更高。因此，99Tcm-d，l-CBPAO有潜力取代99Tcm-d，l-HMPAO成为市售白细胞标记剂。