Ganzalo J A, Jia G Q, Aguirre V, Friend D, Coyle A J, Jenkins N A, Lin G S, Katz H, Lichtman A, Copeland N, Kopf M, Gutierrez-Ramos J C
Center for Blood Research, Incorporated, Boston, Massachusetts 02115, USA.
Immunity. 1996 Jan;4(1):1-14. doi: 10.1016/s1074-7613(00)80293-9.
A model of lung eosinophilia based on the repeated exposure of mice to aerosolized OVA has been used to identify C-C chemokine genes expressed at stages of massive eosinophil infiltration. We describe the identification and cloning of a cDNA that encodes a mouse C-C chemokine with 68% amino acid identity to guinea pig Eotaxin. The recombinant protein encoded by this gene displays potent and specific chemotactic activity for eosinophils, both in vivo and in vitro. Its mRNA levels parallel the kinetics of eosinophil accumulation in the lung during the experimentally induced eosinophilia and it is mainly produced by type I alveolar epithelial cells. The mRNA expression of mouse Eotaxin is not restricted to Th2 T cells in vitro and is independent of the development of a Th2-type response during N. brasiliensis infection, in vivo.
基于小鼠反复暴露于雾化卵清蛋白（OVA）建立的肺部嗜酸性粒细胞增多模型，已被用于鉴定在大量嗜酸性粒细胞浸润阶段表达的C-C趋化因子基因。我们描述了一种cDNA的鉴定和克隆，该cDNA编码一种小鼠C-C趋化因子，与豚鼠嗜酸性粒细胞趋化因子有68%的氨基酸同一性。该基因编码的重组蛋白在体内和体外均对嗜酸性粒细胞显示出强大而特异的趋化活性。其mRNA水平与实验诱导的嗜酸性粒细胞增多症期间肺部嗜酸性粒细胞积累的动力学平行，并且它主要由I型肺泡上皮细胞产生。小鼠嗜酸性粒细胞趋化因子的mRNA表达在体外不限于Th2 T细胞，并且在体内巴西日圆线虫感染期间与Th2型反应的发展无关。
