Hails T, Huttner O, Day A
Biochemistry Department, Oxford University, UK.
Curr Genet. 1995 Oct;28(5):437-40. doi: 10.1007/BF00310812.
We attempted to determine whether Chlamydomonas reinhardtii telomeres, which do not form G-quartet structures readily in vitro, are able to nucleate telomere addition in Saccharomyces cerevisiae. Restricted C. reinhardtii genomic DNA was ligated to a linear S. cerevisiae vector lacking a telomere. A C. reinhardtii telomere ligated to this unprotected end allowed vector replication as a linear DNA molecule in S. cerevisiae. DNA sequencing revealed common [T4AG3]n and variant T6AG3 and T5AG3 C. reinhardtii telomere repeats capped by S. cerevisiae telomere repeat units. The recognition of a C. reinhardtii telomere by the telomere maintenance machinery of S. cerevisiae is consistent with a common theme for telomere structure in organisms with divergent telomere repeats.
莱茵衣藻的端粒在体外不易形成G-四联体结构，我们试图确定其是否能够在酿酒酵母中引发端粒添加。将经过酶切的莱茵衣藻基因组DNA连接到一个缺少端粒的线性酿酒酵母载体上。连接到这个无保护末端的莱茵衣藻端粒使载体能够作为线性DNA分子在酿酒酵母中复制。DNA测序显示，常见的[T4AG3]n以及变体T6AG3和T5AG3莱茵衣藻端粒重复序列被酿酒酵母端粒重复单元所覆盖。酿酒酵母的端粒维持机制对莱茵衣藻端粒的识别，与具有不同端粒重复序列的生物体中端粒结构的共同主题是一致的。
