Sharpe J A, Day A
Genetics Laboratory, Biochemistry Department, Oxford, UK.
Mol Gen Genet. 1993 Feb;237(1-2):134-44. doi: 10.1007/BF00282794.
The first AUG in the Chlamydomonas reinhardtii ADP/ATP translocator (CRANT) mRNA initiates an open reading frame (ORF) which is very similar (51-79% amino acid identity) to other ANT proteins. In contrast to higher plants, no evidence for a long amino-terminal extension was obtained. The 5' non-transcribed region of the single-copy CRANT gene contains sequence motifs present in other C. reinhardtii nuclear genes. Four introns, whose positions are not conserved in other ANT genes, interrupt the protein coding region. A short heat shock specifically reduces CRANT mRNA levels. CRANT mRNA levels were unaffected by a mutation in photosynthesis. In a dark/light regime CRANT mRNA levels are high in the dark phase and low in the early light phase. Data on translation initiation sites, splice junctions and the codon preferences of C. reinhardtii nuclear genes were compiled. With the exception of two rare codons, ACA and GGA, the CRANT gene exhibits the biased codon usage of C. reinhardtii nuclear genes that are highly expressed during normal vegetative growth.
莱茵衣藻ADP/ATP转运体（CRANT）mRNA中的第一个AUG起始一个开放阅读框（ORF），该开放阅读框与其他ANT蛋白非常相似（氨基酸同一性为51 - 79%）。与高等植物不同，未获得存在长氨基末端延伸的证据。单拷贝CRANT基因的5'非转录区包含其他莱茵衣藻核基因中存在的序列基序。四个内含子打断了蛋白质编码区，其位置在其他ANT基因中不保守。短暂的热休克会特异性降低CRANT mRNA水平。光合作用中的突变不影响CRANT mRNA水平。在暗/光周期中，CRANT mRNA水平在黑暗阶段较高，在光照早期较低。汇编了关于莱茵衣藻核基因翻译起始位点、剪接位点和密码子偏好的数据。除了两个稀有密码子ACA和GGA外，CRANT基因表现出在正常营养生长期间高度表达的莱茵衣藻核基因的偏向性密码子使用情况。
