Hadley R D, Miller J D
Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.
Brain Res Dev Brain Res. 1995 Oct 27;89(1):129-38. doi: 10.1016/0165-3806(95)00115-t.
Axonal stumps of cultured Helisoma trivolvis neurons express abundant acetylated microtubules, as a subset of total microtubules. Label completely disappears from the axonal remnants within approximately 1 day, and reappears in newly extended neurites over the course of the next 3-4 days, first in the proximal neurite as short, isolated segments. Acetylated microtubules occur in the neuritic shaft, but never in growth cones or membranous veils. Thus, acetylated microtubules are very labile to the signals generated by axotomy, and their proximodistal re-expression occurs at well separated sites within the neurite as it matures.
培养的三角帆蚌神经元的轴突残端表达大量乙酰化微管，占总微管的一部分。标记在大约1天内从轴突残余物中完全消失，并在接下来的3 - 4天内在新延伸的神经突中重新出现，首先在近端神经突中以短的、孤立的片段形式出现。乙酰化微管出现在神经突轴中，但从未出现在生长锥或膜状面纱中。因此，乙酰化微管对轴突切断产生的信号非常不稳定，并且它们在神经突成熟时在神经突内相隔较远的部位进行近端到远端的重新表达。
