Ferreira A, Cáceres A
Laboratorio de Neurobiología, Instituto de Investigación Médica Mercedes y Martín Ferreyra, Córdoba, Argentina.
Brain Res Dev Brain Res. 1989 Oct 1;49(2):205-13. doi: 10.1016/0165-3806(89)90022-9.
The distribution of acetylated microtubules in cerebellar macroneurons which develop in culture was studied with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. In these neurons 6-11B-1 helps to define a subset of stable, colchicine-resistant, microtubules that during early neuronal development are exclusively localized in the neuron's axon. On the other hand, in mature neurons acetylated microtubules display a widespread distribution being localized in the axon and thick dendritic trunks, a phenomenon correlated with an increase in the levels of acetylated alpha-tubulin and colchicine-resistant microtubules. Taken collectively, these observations suggest that the acetylation of alpha-tubulin is important for differentiating microtubules during neurite growth: in young neurons this post-translational modification may contribute to determine a selective stabilization of microtubules accompanying axonal differentiation.
利用6-11B-1（一种对乙酰化α-微管蛋白具有特异性的单克隆抗体），研究了在培养中发育的小脑大神经元中乙酰化微管的分布情况。在这些神经元中，6-11B-1有助于界定一组稳定的、对秋水仙碱有抗性的微管，在神经元早期发育过程中，这些微管仅定位于神经元的轴突中。另一方面，在成熟神经元中，乙酰化微管分布广泛，定位于轴突和粗大的树突干中，这一现象与乙酰化α-微管蛋白水平和对秋水仙碱有抗性的微管数量增加相关。总体而言，这些观察结果表明，α-微管蛋白的乙酰化对于神经突生长过程中微管的分化很重要：在年轻神经元中，这种翻译后修饰可能有助于确定伴随轴突分化的微管的选择性稳定。
