Almeida A, Brooks K J, Sammut I, Keelan J, Davey G P, Clark J B, Bates T E
Department of Neurochemistry, University of London, UK.
Dev Neurosci. 1995;17(4):212-8. doi: 10.1159/000111289.
The postnatal development of the complexes of the electron transport chain in mitochondria isolated from rat brain synaptosomes was investigated. Synaptosomal brain mitochondria were isolated from rats aged 10-60 days, and the activities of mitochondrial complex I, complex II-III, complex IV and complex V were measured. There was a significant increase in the activity of II-III from day 10 to day 15 and complex IV from day 10 to day 21, thereafter the activities of complexes I-III and IV did not change significantly. The activity of complex I did not change significantly during the period 10-60 days post partum. In synaptic mitochondria, complex V activity was higher than in non-synaptic mitochondria, whereas the activity of complex I was lower than in non-synaptic mitochondria. These data show that the complexes of the respiratory chain within synaptic mitochondria have activities different from those of non-synaptic mitochondria and may have major implications for the relative susceptibility of mitochondria in different brain cell types to neurotoxins such as MPP+, hypoxic/ischaemic damage and oxidative stress.
研究了从大鼠脑突触体分离的线粒体中电子传递链复合体的产后发育情况。从10至60日龄的大鼠中分离出突触体脑线粒体，并测定了线粒体复合体I、复合体II - III、复合体IV和复合体V的活性。从第10天到第15天，II - III的活性显著增加，从第10天到第21天，复合体IV的活性显著增加，此后复合体I - III和IV的活性没有显著变化。产后10 - 60天期间，复合体I的活性没有显著变化。在突触线粒体中，复合体V的活性高于非突触线粒体，而复合体I的活性低于非突触线粒体。这些数据表明，突触线粒体内呼吸链复合体的活性与非突触线粒体不同，这可能对不同脑细胞类型中线粒体对MPP +等神经毒素、缺氧/缺血损伤和氧化应激的相对易感性产生重大影响。
