Srinivasan R, Radjame K, Panicker K N, Dhanda V
Vector Control Research Centre (ICMR), Indira Nagar, Pondicherry, India.
Indian J Exp Biol. 1995 Oct;33(10):757-60.
Oviposition response of gravid P. papatasi females to conspecific eggs was studied in laboratory colonized sandflies. It was observed that significantly higher number of eggs were laid in the vicinity of conspecific eggs. However, a certain minimum number of eggs were required to be placed on the substratum to influence the rate of oviposition. The fecundity of females (mean = 56.5 +/- 4.9 eggs) exposed to conspecific eggs was significantly higher (P < 0.05) than that of blank control group. Perhaps, the chemical substances/pheromone of egg origin stimulated the oviposition rate. In an attempt to isolate the active ingredient, solvent washing of the conspecific eggs were tested. No increase in the rate of oviposition was noticed when the test site was treated with distilled water extract, whereas, significantly larger number of eggs were laid at the site treated with di-ethyl ether extract. This indicated that the oviposition attractant associated with the eggs dissolved in the organic solvent, but not in water. The possible application of this attractant for wild sandflies in nature and for regulating the site of oviposition on the substratum in the laboratory colonized sandflies needs to be explored.
在实验室饲养的白蛉中研究了妊娠雌性巴氏白蛉对同种卵的产卵反应。观察到在同种卵附近产下的卵数量显著更多。然而，需要在基质上放置一定数量的卵才能影响产卵率。暴露于同种卵的雌性的繁殖力（平均 = 56.5 +/- 4.9 个卵）显著高于空白对照组（P < 0.05）。也许，卵源的化学物质/信息素刺激了产卵率。为了分离活性成分，对同种卵进行了溶剂洗涤测试。当测试地点用蒸馏水提取物处理时，未观察到产卵率增加，而在用二乙醚提取物处理的地点产下的卵数量显著更多。这表明与卵相关的产卵引诱剂可溶于有机溶剂，而不溶于水。需要探索这种引诱剂在自然界中对野生白蛉的可能应用以及在实验室饲养的白蛉中调节基质上产卵地点的应用。
