Deppert W, Schirmbeck R
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
Int Rev Cytol. 1995;162A:485-537. doi: 10.1016/s0074-7696(08)61237-1.
Replication of the small DNA tumor virus, simian virus 40 (SV40), is largely dependent on host cell functions, because SV40, in addition to virion proteins, codes only for a few regulatory proteins, the most important one being the SV40 large tumor antigen (T-antigen). This renders SV40 an excellent tool for studying complex cellular and viral processes. In this review we summarize and discuss data providing evidence for virtually all major viral processes during the life cycle of SV40 from viral DNA replication to virion formation, being performed at or within structural systems of the nucleus, in particular the chromatin and the nuclear matrix. These data further support the concept that viral replication in the nucleus is structurally organized and demonstrate that viruses are excellent tools for analyzing the underlying cellular processes. The analysis of viral replication at nuclear structures might also provide a means for specifically interfering with viral processes without interfering with the corresponding cellular functions.
小型DNA肿瘤病毒猿猴病毒40（SV40）的复制很大程度上依赖于宿主细胞功能，因为SV40除了病毒粒子蛋白外，仅编码少数几种调节蛋白，其中最重要的是SV40大T抗原（T抗原）。这使得SV40成为研究复杂细胞和病毒过程的绝佳工具。在本综述中，我们总结并讨论了一些数据，这些数据为SV40生命周期中几乎所有主要病毒过程提供了证据，从病毒DNA复制到病毒粒子形成，这些过程都在细胞核的结构系统内或其内部进行，特别是染色质和核基质。这些数据进一步支持了细胞核内病毒复制是有结构组织的这一概念，并表明病毒是分析潜在细胞过程的优秀工具。对核结构处病毒复制的分析也可能提供一种在不干扰相应细胞功能的情况下特异性干扰病毒过程的方法。
