Nitta I, Ueda T, Watanabe K
Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo.
J Biochem. 1995 Oct;118(4):850-4. doi: 10.1093/oxfordjournals.jbchem.a124990.
We have already reported a novel, in vitro translation system, promoted by pyridine instead of the usual protein factors and energy sources, which consists of only salt-washed ribosomes from Escherichia coli, aminoacyl-tRNA, a template RNA, Na+ and Mg2+ cations, and 40-60% pyridine [Nitta et al. (1994) J. Biochem. 115, 803-807 and the accompanying paper]. Here we show that in this system, pyridine can be replaced not only by pyridine derivatives but also by nucleobases or nucleosides, demonstrating that any compound harboring an aromatic tertiary amine within the molecule possesses such promoting activity. These compounds may serve to assist the peptide bond formation catalyzed by peptidyltransferase within ribosomes. The finding that nucleobases and nucleosides can play such a role in this reaction implies the possibility that these compounds were directly involved in the premordial translation system.
我们已经报道了一种新型的体外翻译系统，该系统由吡啶而非通常的蛋白质因子和能量来源所促进，它仅由来自大肠杆菌的盐洗核糖体、氨酰-tRNA、模板RNA、Na⁺和Mg²⁺阳离子以及40 - 60%的吡啶组成[Nitta等人（1994年）《生物化学杂志》115卷，803 - 807页及相关论文]。在此我们表明，在该系统中，不仅吡啶衍生物可以替代吡啶，核碱基或核苷也可以，这表明分子内含有芳香叔胺的任何化合物都具有这种促进活性。这些化合物可能有助于核糖体中肽基转移酶催化的肽键形成。核碱基和核苷能在该反应中发挥这种作用这一发现意味着这些化合物可能直接参与了原始翻译系统的可能性。
