聚合酶链反应（PCR）在检测1990年至1994年俄罗斯白喉流行期间分离出的产毒白喉棒状杆菌菌株中的应用。
Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994.
作者信息
Mikhailovich V M, Melnikov V G, Mazurova I K, Wachsmuth I K, Wenger J D, Wharton M, Nakao H, Popovic T
机构信息
Russian Federal Reference Laboratory for Diphtheria, Gabrichevsky Institute of Epidemiology and Microbiology, Moscow, Russia.
出版信息
J Clin Microbiol. 1995 Nov;33(11):3061-3. doi: 10.1128/jcm.33.11.3061-3063.1995.
Abstract
A total of 250 Corynebacterium diphtheriae isolates from clinical cases and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigenic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete concordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinical laboratories.
摘要
运用针对白喉毒素基因A亚基的聚合酶链反应（PCR），对来自俄罗斯临床病例和带菌者的总共250株白喉棒状杆菌分离株进行检测，以区分产毒菌株和非产毒菌株；170株菌株因存在248碱基对的扩增子而呈阳性。该PCR检测结果与标准免疫沉淀检测（Elek）结果完全一致，且PCR检测是临床实验室快速鉴定的有用工具。
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