携带白喉毒素基因的非产毒白喉棒状杆菌菌株

[Corynebacterium diphtheriae nontoxigenic strain carrying the gene of diphtheria toxin].

作者信息

Mel'nikov V G, Kombarova S Iu, Borisova O Iu, Volozhantsev N V, Verevkin V V, Volkovoĭ K I, Mazurova I K

机构信息

Gabrichevsky Research Institute of Epidemiology and Microbiology, Moscow, Russia.

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2004 Jan-Feb(1):3-7.

PMID:15024972

Abstract

Among 828 C. diphtheriae nontoxigenic cultures isolated in different region of Russia in 1994-2002, 114 cultures (13.8%) had the gene of diphtheria toxin (gene tox) and were thus called nontoxigenic tox-carrying (NTTC) strains. All NTTC strains were found to belong to biovar mitis and formed neither normal, nor "defective" diphtheria toxin. The most of NTTC strains (94%) belonged to ribotype "Moskva", not occurring among C. diphtheriae toxigenic strains. The incapacity of NNTC strains of forming diphtheria toxin was caused by mutation: the deletion of one nucleotide which led to the shift of the open reading frame and to the formation of the stop codon. The results of these studies are indicative of the fact that a sufficiently homogeneous and isolated group of C. diphtheriae nontoxigenic strains is spread in Russia. These strains carry the nonexpressing gene of diphtheria toxin and are of no epidemic importance in diphtheria infection.

摘要

在1994 - 2002年俄罗斯不同地区分离出的828株非产毒白喉棒状杆菌培养物中，有114株培养物（13.8%）含有白喉毒素基因（tox基因），因此被称为非产毒携毒（NTTC）菌株。所有NTTC菌株均属于轻型生物变种，既不产生正常的，也不产生“缺陷型”白喉毒素。大多数NTTC菌株（94%）属于“莫斯科”核糖型，在产毒白喉棒状杆菌菌株中未出现。NTTC菌株不能产生白喉毒素是由突变引起的：一个核苷酸的缺失导致开放阅读框移位并形成终止密码子。这些研究结果表明，在俄罗斯存在一个足够同质且孤立的非产毒白喉棒状杆菌菌株群体。这些菌株携带无表达功能的白喉毒素基因，在白喉感染中不具有流行病学重要性。