Heimer R, Bashey R I, Kyle J, Jimenez S A
Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.
J Mol Cell Cardiol. 1995 Oct;27(10):2191-8. doi: 10.1016/s0022-2828(95)91479-x.
In this study we examined the production of proteoglycans by fibroblasts cultured from the left ventricular myocardium of normal adult rats. Various molecular species of proteoglycan were detected, either by labeling glycosaminoglycan chains with 35SO4 or by labeling the proteoglycan core protein with [35S]methionine. The medium of the cell cultures, which contained quantitatively most of the proteoglycans, appeared to consist mainly of biglycan, lesser amounts of decorin and proteoglycans of higher molecular weight. Biglycan and decorin were identified not only by the characteristic mobility of the intact protein and the core protein but also by immunolocation on Western blots. TGF-beta upregulated the synthesis of all these proteoglycans, coincident with elongation of glycosaminoglycan side chains observed for biglycan and decorin. The apparent molecular weight of the core protein of the two proteoglycans remained unaffected by TGF-beta. The results of these experiments suggest that with regard to proteoglycan synthesis and its regulation by TGF-beta, cultured fibroblasts originating from the myocardium share to a large extent the properties of cultured fibroblasts of other organs.
在本研究中，我们检测了从正常成年大鼠左心室心肌培养的成纤维细胞产生蛋白聚糖的情况。通过用35SO4标记糖胺聚糖链或用[35S]甲硫氨酸标记蛋白聚糖核心蛋白，检测到了各种分子种类的蛋白聚糖。细胞培养物的培养基中含有定量的大部分蛋白聚糖，其似乎主要由双糖链蛋白聚糖组成，还有少量的饰胶蛋白聚糖和更高分子量的蛋白聚糖。双糖链蛋白聚糖和饰胶蛋白聚糖不仅通过完整蛋白和核心蛋白的特征迁移率来鉴定，还通过蛋白质印迹法上的免疫定位来鉴定。转化生长因子-β（TGF-β）上调了所有这些蛋白聚糖的合成，这与双糖链蛋白聚糖和饰胶蛋白聚糖观察到的糖胺聚糖侧链延长相一致。两种蛋白聚糖核心蛋白的表观分子量不受TGF-β的影响。这些实验结果表明，就蛋白聚糖合成及其受TGF-β的调节而言，源自心肌的培养成纤维细胞在很大程度上具有其他器官培养成纤维细胞的特性。
