Shattuck K E, Bhatia J, Grinnell C, Rassin D K
Department of Pediatrics, University of Texas Medical Branch, Galveston 17555-0526, USA.
JPEN J Parenter Enteral Nutr. 1995 Sep-Oct;19(5):398-402. doi: 10.1177/0148607195019005398.
Administration of parenteral nutrition (PN) that has been irradiated with light is associated with hepatic dysfunction in rats in vivo. Using the isolated perfused rat liver, we report the in vitro hepatic response to a light-exposed amino acid-vitamin (AAV) solution, compared with a light-protected solution.
The amino acid-vitamin solution (3 g Aminosyn and 2.5 mL MVI-12 added to buffer) was placed under a lamp in a beaker that was covered completely with foil (light-protected) or with a transparent wrap (light-exposed) for 24 hours before liver perfusion. Livers from adult male rats were isolated and perfused with buffer for 30 minutes, with the AAV solution for 60 minutes, and again with buffer for 30 minutes.
Infusion with the AAV solution resulted in decreases in bile flow rates. Compared with light-protected groups, light-exposure was associated with significantly lower bile flow rates, significant increases in biliary concentrations of oxidized glutathione (GSSG), and significantly decreased biliary concentrations of free amino acids, including the glutathione precursors glutamate and glycine.
Perfusion of the isolated rat liver with an AAV solution that has been irradiated with light for 24 hours results in a decrease in bile flow rates and an increase in biliary GSSG concentrations, suggesting oxidant stress. Consideration should be given to protecting solutions from light in the clinical setting.
给大鼠体内输注经光照处理的肠外营养（PN）会导致肝功能障碍。我们使用离体灌注的大鼠肝脏，报告了与避光保存溶液相比，肝脏对光照处理的氨基酸 - 维生素（AAV）溶液的体外反应。
在肝脏灌注前24小时，将氨基酸 - 维生素溶液（3 g Aminosyn和2.5 mL MVI - 12加入缓冲液中）置于烧杯中，烧杯用铝箔完全覆盖（避光）或用透明包装膜覆盖（光照），然后放在灯下。分离成年雄性大鼠的肝脏，先用缓冲液灌注30分钟，再用AAV溶液灌注60分钟，然后再次用缓冲液灌注30分钟。
输注AAV溶液导致胆汁流速降低。与避光组相比，光照组的胆汁流速显著降低，氧化型谷胱甘肽（GSSG）的胆汁浓度显著升高，包括谷胱甘肽前体谷氨酸和甘氨酸在内的游离氨基酸的胆汁浓度显著降低。
用经24小时光照处理的AAV溶液灌注离体大鼠肝脏会导致胆汁流速降低和胆汁GSSG浓度升高，提示存在氧化应激。临床环境中应考虑对溶液进行避光处理。
