Suzutani T, Koyano S, Takada M, Yoshida I, Azuma M
Department of Microbiology, Asahikawa Medical College, Hokkaido, Japan.
Microbiol Immunol. 1995;39(10):787-94. doi: 10.1111/j.1348-0421.1995.tb03271.x.
The virulence of thymidine kinase-negative herpes simplex virus type 1 (HSV-1; VRTK- strain) and type 2 (HSV-2; UWTK- strain) was studied in comparison with that of their parental strains (VR-3 and UW-268, respectively) in an encephalitis model of adult (4-week-old) and newborn (3-day-old) mice. Viral thymidine kinase (TK) activity was essential for the maximum expression of virulence of HSV-1, because the 50% lethal dose (LD50) of VRTK- was 60 times higher than that of VR-3 in the brains of newborn mice expressing high levels of cellular TK activity. However, the UWTK- strain showed that replication of the UWTK- strain was completely supported by cellular TK activity. This difference in the role of viral and cellular TKs for virus growth between HSV-1 and HSV-2 was¿ confirmed with the one-step growth of virus strains in L-M and L-M(TK-) cells.
在成年（4周龄）和新生（3日龄）小鼠的脑炎模型中，研究了胸苷激酶阴性单纯疱疹病毒1型（HSV-1；VRTK-株）和2型（HSV-2；UWTK-株）与其亲本株（分别为VR-3和UW-268）相比的毒力。病毒胸苷激酶（TK）活性对于HSV-1毒力的最大表达至关重要，因为在表达高水平细胞TK活性的新生小鼠脑中，VRTK-的50%致死剂量（LD50）比VR-3高60倍。然而，UWTK-株表明UWTK-株的复制完全由细胞TK活性支持。HSV-1和HSV-2之间病毒和细胞TK在病毒生长中的作用差异通过病毒株在L-M和L-M（TK-）细胞中的一步生长得到证实。
