一种用于鉴定食品样本中李斯特菌的简单菌落印迹法。
A simple colony-blot method for identification of Listeria in food samples.
作者信息
Belyi Y F, Varfolomeeva N A, Tartakovskii I S
机构信息
N.F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
出版信息
Med Microbiol Immunol. 1995 Oct;184(3):105-8. doi: 10.1007/BF00224345.
A method for identification of Listeria in food samples was developed. It consisted of cultivating of suspected specimen on standard agar medium, direct absorption of grown colonies onto nitrocellulose membrane and processing of the latter with rabbit serum raised against purified cell wall protein Lm79/39 of L. monocytogenes. Analysis using anti-rabbit peroxidase conjugate and 4-chloro-1-naphthol and H2O2 solutions allowed direct detection of Listeria colonies which remained readily available for subsequent isolation.
开发了一种用于鉴定食品样本中李斯特菌的方法。该方法包括在标准琼脂培养基上培养疑似标本，将生长的菌落直接吸附到硝酸纤维素膜上，并用针对单核细胞增生李斯特菌纯化细胞壁蛋白Lm79/39产生的兔血清处理后者。使用抗兔过氧化物酶结合物以及4-氯-1-萘酚和过氧化氢溶液进行分析，可以直接检测到李斯特菌菌落，这些菌落仍易于用于后续分离。
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