McClain D, Lee W H
U.S. Department of Agriculture, Food Safety and Inspection Service, Beltsville, MD 20705.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):660-4.
A method was developed specifically to detect naturally occurring Listeria monocytogenes in meat because the traditional cold enrichment procedure was extremely slow and other procedures were ineffective. This method could identify beta-hemolytic Listeria colonies in 3-4 days. The use of a 2-stage enrichment, highly selective LPM agar, and a thin-layer horse blood agar plate for the detection of beta-hemolytic Listeria isolates are the important steps of this method. L. monocytogenes was recovered from 20 of 41 samples of frozen ground beef, 12 of 23 samples of pork sausage, and 7 of 22 samples of poultry. These results indicate that L. monocytogenes is common in raw meat and that this method is effective for its recovery.
开发了一种专门用于检测肉类中天然存在的单核细胞增生李斯特菌的方法，因为传统的冷增菌程序极其缓慢，而其他程序无效。该方法可在3-4天内鉴定出β-溶血李斯特菌菌落。采用两阶段增菌、高选择性LPM琼脂和薄层马血琼脂平板检测β-溶血李斯特菌分离株是该方法的重要步骤。从41份冷冻碎牛肉样本中的20份、23份猪肉香肠样本中的12份和22份家禽样本中的7份中分离出了单核细胞增生李斯特菌。这些结果表明，单核细胞增生李斯特菌在生肉中很常见，并且该方法对其回收有效。
