Nilsen T W
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Mol Biochem Parasitol. 1995 Jul;73(1-2):1-6. doi: 10.1016/0166-6851(94)00107-x.
5'-end maturation of messenger RNAs via acquisition of a trans-spliced leader sequence occurs in several primitive eukaryotes, some of which are parasitic. This type of trans-splicing proceeds though a two-step reaction pathway directly analogous to that of cis-splicing and like cis-splicing it requires multiple U snRNP cofactors. This minireview attempts to provide a brief synopsis of our current understanding of the evolution and biological significance of trans-splicing. Progress in deciphering the mechanism of trans-splicing, particularly as it relates to current models of cis-splicing, is also discussed.
信使核糖核酸通过获取一个反式剪接前导序列进行5'端成熟过程，这发生在几种原始真核生物中，其中一些是寄生生物。这种类型的反式剪接通过一个两步反应途径进行，该途径与顺式剪接的途径直接类似，并且和顺式剪接一样，它需要多个U小核核糖核蛋白辅助因子。这篇小型综述试图简要概述我们目前对反式剪接的进化和生物学意义的理解。还讨论了在破译反式剪接机制方面取得的进展，特别是与当前顺式剪接模型相关的进展。
