[同一细胞中核酸和蛋白质组分的序列展示]
[Sequential demonstration of nucleic acids and protein fractions in the same cells].
作者信息
Erenpreĭsa E A
出版信息
Arkh Anat Gistol Embriol. 1977 Feb;72(2):95-7.
Some combinations are suggested to reveal cell components in the following sequence: 1) RNA--DNA--total proteins; 2) RNA--DNA--histones; 3) RNA--DNA--histones--total proteins; 4) RNA--DNA--acidic proteins. RNA is stained with pyronin Y (in the mixture with methyl green). DNA--by the Feulgen procedure (with TCA hydrolysis), histones and total proteins--by Alfert--Geschwind's method and acidic proteins--according to the technique of Smetana and Bush. The details of the whole procedure, adopted for formol-fixed smears are described.
建议采用以下顺序的某些组合来显示细胞成分
1）RNA—DNA—总蛋白；2）RNA—DNA—组蛋白；3）RNA—DNA—组蛋白—总蛋白；4）RNA—DNA—酸性蛋白。RNA用派洛宁Y（与甲基绿混合）染色。DNA采用福尔根法（经三氯乙酸水解）染色，组蛋白和总蛋白采用阿尔费特—格施温德法染色，酸性蛋白则根据斯梅塔纳和布什的技术进行染色。文中描述了用于甲醛固定涂片的整个操作细节。
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