Erenpreĭsa E A
Arkh Anat Gistol Embriol. 1977 Feb;72(2):95-7.
Some combinations are suggested to reveal cell components in the following sequence: 1) RNA--DNA--total proteins; 2) RNA--DNA--histones; 3) RNA--DNA--histones--total proteins; 4) RNA--DNA--acidic proteins. RNA is stained with pyronin Y (in the mixture with methyl green). DNA--by the Feulgen procedure (with TCA hydrolysis), histones and total proteins--by Alfert--Geschwind's method and acidic proteins--according to the technique of Smetana and Bush. The details of the whole procedure, adopted for formol-fixed smears are described.
1)RNA—DNA—总蛋白;2)RNA—DNA—组蛋白;3)RNA—DNA—组蛋白—总蛋白;4)RNA—DNA—酸性蛋白。RNA用派洛宁Y(与甲基绿混合)染色。DNA采用福尔根法(经三氯乙酸水解)染色,组蛋白和总蛋白采用阿尔费特—格施温德法染色,酸性蛋白则根据斯梅塔纳和布什的技术进行染色。文中描述了用于甲醛固定涂片的整个操作细节。