Benzodiazepines and palatability: taste reactivity in normal ingestion.

The taste reactivity (TR) test was devised as a method to obtain behavioural data in response to gustatory stimuli in neurologically impaired rats, incapable of voluntary feeding. Sapid solutions were infused through surgically implanted intraoral cannulae. Facial and motor responses corresponded well to known hedonic and aversive properties of tastes (e.g., sweet, bitter). TR testing has since proved effective as an adjunct to intake-based methods, in the psychopharmacology of ingestion in the normal rat. We developed a nonsurgical modification of the TR test, in which intact rats sampled stimuli voluntarily. The benzodiazepine receptor agonist midazolam (3.0 mg/kg, IP) was administered to rats first trained to consume a sweet 3% sucrose solution, and later tested with access to a bitter 0.01% quinine solution. Response were videotaped, and TR measures were scored during periods of noningestion using a frame-by-frame playback. Treatment increased ingestion and facilitated ingestive responses in accordance with published data for cannulated rats. Results support a two-component view of response palatability, in which treatment alters feeding motivation, increasing positive palatability and facilitating ingestion of both palatable and unpalatable stimuli.