Otsuji E, Yamaguchi T, Kodama T, Takahashi T
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Tohoku J Exp Med. 1995 Aug;176(4):213-8. doi: 10.1620/tjem.176.213.
Histologic diagnosis of poorly differentiated gastric adenocarcinomas by conventional hematoxylin-eosin (HE) staining is often limited by the quality of gastroendoscopic biopsy specimens. In this study we attempted to identify gastric cancer cells in biopsy specimens and ascites of gastric cancer patients using the gastric cancer reactive monoclonal antibodies S202 and S74. Twenty-four of 25 specimens shown to contain cancer cells by HE staining were stained positively by monoclonal antibody S202. In contrast, 3 of 16 specimens that were diagnosed as not including cancer cells by HE staining was stained positively by S202. The possibility that cancer cells in the biopsy specimens were not detected by HE staining cannot be ruled out. Cancer cells in ascites of patients diagnosed with carcinomatous peritonitis by Papanicolaou staining were stained positively by monoclonal antibody S74. From these results, we conclude that monoclonal antibodies S202 and S74 may be useful supplemental agents for the diagnosis of gastric cancer.
通过传统苏木精 - 伊红（HE）染色对低分化胃腺癌进行组织学诊断常常受到胃镜活检标本质量的限制。在本研究中，我们尝试使用胃癌反应性单克隆抗体S202和S74来识别胃癌患者活检标本和腹水中的胃癌细胞。25份经HE染色显示含有癌细胞的标本中有24份被单克隆抗体S202阳性染色。相比之下，16份经HE染色诊断为不包含癌细胞的标本中有3份被S202阳性染色。不能排除活检标本中的癌细胞未被HE染色检测到的可能性。经巴氏染色诊断为癌性腹膜炎患者腹水中的癌细胞被单克隆抗体S74阳性染色。从这些结果来看，我们得出结论，单克隆抗体S202和S74可能是诊断胃癌有用的辅助试剂。
