Olsson M L, Chester M A
Blood Centre, University Hospital, Lund, Sweden.
Vox Sang. 1995;69(3):242-7. doi: 10.1111/j.1423-0410.1995.tb02602.x.
An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
通过发现仅存在于B和O2等位基因中的一种新型核苷酸取代（G1096A），一种能够区分ABO位点常见等位基因A1、A2、B、O1和O2的ABO基因型筛查方法成为可能。我们开发了一种快速可靠的单管方法，该方法使用多重PCR，用四种引物扩增ABO基因的第6和第7外显子，随后同时添加两种限制性内切酶，并在150名瑞典献血者群体中进行了验证。该技术是迄今为止报道的最具成本效益且信息丰富的ABO基因分型方法。
