Setyono B, Grossmann M, Liautard J P
Biochimie. 1977;59(1):43-9. doi: 10.1016/s0300-9084(77)80084-9.
The cytoplasm of HeLa cells contains a series of proteins with affinity for RNA. These proteins were detected by affinity chromatography on RNA-Sepharose. Several proteins are tightly bound to RNA; there is no preference for mRNA as the same proteins bind to any free RNA, even poly(A). At least two of these proteins coincide with proteins of genuine ps-mRNP-proteins (Mr. 50 000 and 75 000). The column yields a protein (Mr. 37 000) which has an affinity for the matrix rather than for the coupled RNA. Complexes formed from mRNA and cytosol proteins do not bind to ribosomes, whereas ps-mRNPs does. We suggest that some of the polysomal mRNP proteins pre-exist unbound in the cytosol.
海拉细胞的细胞质中含有一系列对RNA具有亲和力的蛋白质。这些蛋白质通过在RNA-琼脂糖上的亲和层析法检测到。有几种蛋白质与RNA紧密结合；对mRNA没有偏好，因为相同的蛋白质能与任何游离RNA结合，甚至是聚腺苷酸。这些蛋白质中至少有两种与真正的多核糖体mRNA结合蛋白（分子量50000和75000）一致。该柱产生一种对基质而非偶联RNA具有亲和力的蛋白质（分子量37000）。由mRNA和胞质溶胶蛋白形成的复合物不与核糖体结合，而多核糖体mRNA结合蛋白复合物则能结合。我们认为，一些多核糖体mRNA结合蛋白在胞质溶胶中以未结合的形式预先存在。
